Cryopreservation of reproductive entities is gaining a constantly growing importance. What used to be a means for storage of semen and embryos now has a much wider application. On top of being part of ongoing subfertility treatment, it now also plays an important role in extension and preservation of fertility.We see an increase in cumulative pregnancy rate, a possibility of single embryo transfer, a less troublesome second treatment, a possibility to deal with legal restrictions (e.g. Italy), and a number of practical issues. We have the possibility of extending and preserving fertility of health reasons, e.g. cancer and premature menopause, and of social and personal reasons varying from country to country.With more or less success, the embryologists are now able to preserve all types of reproductive structures – from gonadal tissue and immature oocytes to hatched blastocysts. Still there are only two main methods – slow freezing and vitrification.Royan Vitrification Workshop was held on August 26th, in cooperation with Medicult Company. This workshop and was aimed to touch on slow freezing, but mainly looked at various aspects of vitrification: What is vitrification, which tools are used, how is it done, and how successful is it?